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TOV-21G 人卵巢癌細(xì)胞

發(fā)布時(shí)間：2016-08-01瀏覽次數(shù)：1821返回列表

Designations:

TOV-21G

Depositors:

University of Montreal

Biosafety Level:

Shipped:

frozen

Medium & Serum:

See Propagation

Growth Properties:

adherent

Organism:

Homo sapiens deposited as human

Morphology:

epithelial

Source:

Organ: ovary
Tumor Stage: grade 3, stage III
Disease: primary malignant adenocarcinoma; clear cell carcinoma

Cellular Products:

keratin

Permits/Forms:

In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications:

Like OV-90 (ATCC CRL-11732 ), the OV-21G (ATCC CRL-11730 ) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731 ) cell line does not exhibit a deletion at chromosome 3p24.

Tumorigenic:

Yes

Oncogene:

p53 + (wild type)

DNA Profile (STR):

Amelogenin: X
CSF1PO: 13,15
D13S317: 11,12
D16S539: 10,12
D5S818: 12,13
D7S820: 12
THO1: 7,9.3
TPOX: 8,11
vWA: 17

Cytogenetic Analysis:

47, XX, +10 [49408 ]

Age:

62 years

Gender:

female

Comments:

This cell line was initiated in October of 1991 from a patient of French-Canadian descent with no family history of ovarian cancer.
Like OV-90 (ATCC CRL-11732 ), the OV-21G (ATCC CRL-11730 ) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731 ) cell line does not exhibit a deletion at chromosome 3p24.

Propagation:

ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 15%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C

Subculturing:

Protocol:

Remove and discard culture medium.
Briefly rinse the cell layer with 0.05% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended
Medium Renewal: Every 3 to 4 days

Preservation:

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase

Related Products:

recommended serum:ATCC 30-2020

References:

42090: Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998
49408: Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

CoC1	人卵巢癌細(xì)胞系	Lec1	倉鼠卵巢細(xì)胞
A2780	人卵巢癌細(xì)胞	OVAC	卵巢癌細(xì)胞
Anglne	人卵巢癌細(xì)胞系	NIH:OVCAR-3	人卵巢癌細(xì)胞
Caov－3	人乳突狀卵巢腺癌細(xì)胞	PA-1	人卵巢畸胎瘤細(xì)胞
CHO	倉鼠卵巢細(xì)胞	Pr-HNV8	菜青蟲卵巢細(xì)胞
CHO/dhFr-	倉鼠卵巢細(xì)胞,二氫葉酸還原酶缺陷	RP70-a-CHO-0.8MTX	倉鼠卵巢細(xì)胞
CHO-K1	倉鼠卵巢細(xì)胞	SF9	晚秋粒蟲卵巢細(xì)胞
CHO-pt	轉(zhuǎn)血小板基因倉鼠卵巢細(xì)胞	SKOV3	人卵巢癌細(xì)胞
CHO-S	倉鼠卵巢懸浮細(xì)胞	SKOV-3/DDP	卵巢癌順鉑耐藥株
CoC1	人卵巢癌細(xì)胞系	SW1910	人卵巢癌細(xì)胞
CoC2	卵巢癌	SW626	人卵巢腺癌細(xì)胞
CTLA4 Ig-24	倉鼠卵巢細(xì)胞	TOV-21G	人卵巢癌
ES-2	人卵巢透明細(xì)胞癌	ZA1	轉(zhuǎn)S9基因倉鼠卵巢細(xì)胞
HO-8910	人卵巢癌細(xì)胞	ZA6	轉(zhuǎn)S9基因倉鼠卵巢細(xì)胞
HO-8910PM	人高轉(zhuǎn)移卵巢癌細(xì)胞	Cho-K1	倉鼠卵巢細(xì)胞亞株
HOC1	卵巢癌細(xì)胞	SK-OV-3	人卵巢癌細(xì)胞
Lec1	倉鼠卵巢細(xì)胞	Sf9	昆蟲卵巢細(xì)胞
CHO-K1	倉鼠卵巢細(xì)胞亞株	ES-2	人卵巢透明細(xì)胞癌
CHO/dhFr-	倉鼠卵巢細(xì)胞,二氫葉酸還原酶缺陷	SK-OV-3	人卵巢癌細(xì)胞TOV-21G 人卵巢癌細(xì)胞TOV-21G 人卵巢

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TOV-21G 人卵巢癌細(xì)胞