其他原核表達(dá)載體PBBR1 MCS-3 Tet+，PBBR1 MCS-4 A+

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下面所有質(zhì)粒和菌株，均為本公司實(shí)驗(yàn)室親自驗(yàn)證過，可以提供技術(shù)支持，載體來源于promega,addgene,clontech,可提供質(zhì)粒圖譜，可提供近萬種質(zhì)粒載體、菌株、基因和細(xì)胞株,并可提供實(shí)驗(yàn)技術(shù)外包服務(wù),如基因克隆、質(zhì)粒構(gòu)建、蛋白原核、真核表達(dá)及純，pQE 載體，原核表達(dá)載體，克隆載體 ，畢赤酵母表達(dá)載體，畢赤酵母宿主菌，釀酒酵母表達(dá)載體 ，釀酒酵母宿主菌，真菌表達(dá)載體，真核哺乳動(dòng)物熒光載體，真核哺乳動(dòng)物載體，植物表達(dá)載體，大腸桿菌宿主菌，枯草宿主菌

釀酒酵母表達(dá)載體

pYES2,pYES2/NT,pYES2/CT,pYES3,pYES6, pYCplac22-GFP,酵母載體pAUR123,pRS303TEF,pRS304, pRS305,pRS306,pY13TEF,pY14TEF，pY15TEF，pY16TEF,酵母基因重組表達(dá)載體pUG6, pSH47,酵母單雜載體pHISi,pLacZi,pHIS2, pGAD424, 酵母雙雜交系統(tǒng):釀酒酵母Y187,釀酒酵母AH109；質(zhì)粒pGADT7,pGBKT7；對照質(zhì)粒pGBKT7-53，pGBKT7-lam，pGADT7-T，PCL1，釀酒酵母菌株INVSc1,YM4271, AH109,Y187,Y190, 畢赤酵母表達(dá)載體pPIC9K,pPIC9K-His,pPIC3.5K,pPICZalphaA,B,C,pPICZA,B,C,pGAPZαA,pAO815,pPIC9k-His,pHIL-S1,pPink hc，配套畢赤酵母Pichiapink,畢赤酵母宿主X33，KM71，KM71H，GS115，

原核表達(dá)載體pQE30,31,32,40,60,61,62,等原核表達(dá)載體,包括pET系列，pET-GST，pGEX系列(含GST標(biāo)簽)，pMAL系列pMAL-c2x,-c4x,-c4e,-c5x,-p5x,pBAD,pBADHis,pBADmycHis系列，pQE系列,pTrc99a,pTrcHis系列，pBV220,221,222,pTXB系列，pLLP-ompA,pIN-III-ompA(分泌型表達(dá)系列),pQBI63(原核表達(dá)帶熒光)pET3a, pET 3d, pET 11a, pET 12a, pET 14b, pET 15b, pET 16b, pET 17b, pET 19b, pET 20b, pET 21a,b,d, pET 22b, pET 23a, pET 23b, pET 24a,b, pET 25b, pET 26b, pET 27b, pET 28a,b, pET 29a, pET 30a, pET 31b, pET 32a, pET 35b, pET 38b, pET 39b, pET 40b, pET 41a,b pET 42a, pET 43.1a,b pET 44a, pET 49b pET302,303 pET His,pET Dsb,pET GST,pET Trx pQE2, pQE9 pQE30,31,32, pQE 40 pQE70 pQE80L pQETirs system pRSET-A pRSET-B pRSET-C pGEX4T-1,-2,-3,5x-1,6p-1,6p-2,2tk,3c pBV220,221,222 pTrcHisA,B,C pBAD24,34,43 pBAD HisA,B,C pPinPoint-Xa1,Xa2,Xa3 pMALc2x, p2x pBV220 pGEM Ex1, pGEM7ZF(+), pTrc99A, pTwin1, pEZZ18 pkk232-8,pkk 233-3,pACYC184,pBR322,pUC119 pTYB1,pTYB2,pTYB4,pTYB11 pBlueScript SK(+),pBlueScript SK(-) pLLP ompA, pINIIIompA, pMBP-P ,pMBP-C, 大腸桿菌冷激質(zhì)粒： pColdI pColdII pColdIII pColdTF 原核共表達(dá)質(zhì)粒：pACYCduet-1,pETduet-1,pCDFduet-1，pRSFduet-1 Takara公司大腸桿菌分子伴侶： pG-KJE8 pGro7 pKJE7 pGTf2 pTf16 大腸桿菌宿主細(xì)胞： DH5a JM101 JM103 JM105 JM107 JM109 JM110 0 0F BL21(DE3) HB101 ER2529 E2566 C2566 MG1655 XL-10gold XL blue M15 JF1125 K802 SG1117 BL21(AI) BL21(DE3)plysS TG1 TB1 DH5a(pir) Tuner(DE3) Bl21 codonplusRIPL Novablue(DE3) Rosetta Rosetta(DE3) Rosetta(DE3)plys Rosetta-gami(DE3) Rosetta-gamiB(DE3), Rosetta-gamiB(DE3)plysS Orgami(DE3) OrgamiB(DE3) HMS174(DE3)

植物表達(dá)/RNAi載體農(nóng)桿菌pBI121,pBI121-GFP,pBI101,pBI221,pSN1301, pUN1301,pRTL2 , pRTL2-GFP , pRTL2-CFP, pRTL2-RFP , pRTL2-YFP,pCAMBIA 1300, 1301, 1302,1303,1304,1305, 1381Z,1391Z,2300, 2301,3300,3301,pCAMBIA super1300,pCAMBIA super1300-GFP,pPZP212,pPZP2121,pPZP212-GFP,pGDG,RNAi載體pART27,pHANNIBAL,pKANNIBAL, pFGC5941,pTCK303, pTRV1,pTRV2, T-DNA插入載體(隨機(jī)突變體庫)pSKI015,pSKI074,真菌ATMT載體pBIG2RHPH2-GUS-GFP,pBHt1

枯草芽孢桿菌表達(dá)載體pWB980,pHT43,pHP13,pHP43, pBE2,pMUTIN4,pUB110,pE194,pMA5, pMK3,pMK4,pHT304,pHY300PLK, pBest502,pDG1363,pSG1154,pAX01, pSAS144,pDL,pDG148-stu,pDG641, pAL12,pUCX05-bgaB,pHT01， 配套菌株BS 168,WB600,WB800,WB700, WB800N,1012,FZB42,1A747,廣宿主質(zhì)粒pVLT33

RNAi基因沉默干擾敲除載體pSilencer1.0，pSilencer 2.1-U6 hygro， pSilencer 3.1-H1 hygro，pSilencer 3.1-H1 neo， pSilencer 4.1-CMV neo， pSilencer 4.1-CMV puro pMIR-REPORT Luciferase RNAi載體(oligoengine) pSuper-puro RNAi逆轉(zhuǎn)錄病毒載體(clontech): RNAi-Ready pSIREN-Retro Q， RNAi-Ready pSIREN-RetroQ-ZsGreen(Luciferase shRNA Annealed Oligonucleotide) RNAi慢病毒載體(addgene): pLKO.1

基因cDNA文庫ORF表達(dá)克隆質(zhì)粒，

擁有目前zui大的cDNA克隆庫、覆蓋10萬人、鼠全長基因的cDNA克隆和超過25,000個(gè)ORF克隆表達(dá)載體。

哺乳動(dòng)物表達(dá)載體pcDNA3.1+/-,pcDNA4/HisMax B，pSecTag2 A，pVAX1，pBudCE4.1,pTracer CMV2，pcDNA3.1(-)/myc-His A ，pcDNA6-Myc/His B，pCEP4， pIRES，pIRESneo，pIRES hyg3，pCMV-myc，pCMV-HA，pIRES-puro3，pIRES-neo3,pCAGGS哺乳動(dòng)物雙雜交系統(tǒng)pACT，pBIND，pACT-MyoD，pBIND-Id，pG5luc,pCMV-BD, pCMV-AD, pBD-p53, pFR-luc,Cytotrap Two-Hybrid System:pSos, pSos MAFB, pMyr蛻皮激素誘導(dǎo)系統(tǒng)pIND, pVgRxR,LacSwith II哺乳動(dòng)物誘導(dǎo)表達(dá)系統(tǒng):pOPRSVI ，pOPI3CAT，pCMVLacI,GeneSwitch System:pSwitch哺乳動(dòng)物表面展示系統(tǒng):pDisplay, 四環(huán)素調(diào)控系統(tǒng)(Invitrogen)：pcDNA4/TO/Myc-His A，pcDNA4/TO/Myc-His B，pcDNA4/TO/Myc-His C，pcDNA4/TO/Myc-His/LacZ，pcDNA6/TR四環(huán)素調(diào)控系統(tǒng)(Clontech)：pTet-On，pTet-Off，pTRE2,pRevTRE，pRevTet-On，pRevTet-off信號通路報(bào)告載體:pGAS-TA-Luc，pSTAT3-TA-Luc, pISRE-TA-Luc, pTA-Luc，pIκB-EGFP，pNFAT-TA-Luc，pCaspase3-sensor，pAP1(PMA)-Luc;pGL4.26[luc2P/minP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFAT-RE/Hygro]，pGL4.75;p53-Luc，pAP-1-Luc， pNF-κB-Luc，pSRE-Luc，pFA2-Elk1，pFC-MEKK，pFR-luc,Gateway系統(tǒng)(invitrogen)pcDNA6.2-GWEmGFP-miR negative, pLenti 6/TR,pcDNA 6.2-GW EmGFP-miR，

乳酸菌表達(dá)載體及各種乳酸菌乳酸桿菌菌株，pNZ8148,pLEISS,pMG36e,pBBR1MCS-5,pBBR1MCS-6,pRV610,pLEM415,pHY300PLK,分泌型乳酸菌表達(dá)載體pVE5523，pPG611.1,pPG612.1等和乳酸桿菌菌株宿主菌NZ9000,MG1363,Lactobacillus casei 1.539,Lactobacillus casei,acidophilus NCFM,1.2,Lactobacillus sakei 23K,L.plantarum,L.rhamnosus GG,B.coagulans,Bifidobacterium bifidum,Bifidobacterium infantis,Lactococcus lactis M17,1663,Lactobacillus reuterii

廣宿主表達(dá)載體鏈球菌表達(dá)敲除載體假單胞菌表達(dá)載體pVLT33,pBBR1MCS-2,3,4,5,6, pJRD215,pJN105,pME6032,Cos載體pLAFR3,pMP2444(GFP), pHY300PLK,pRT102,pRL1063a, 轉(zhuǎn)座子載體pUT-miniTn5,pMGS100, pWHM10,pKC1139,pSET152, pOJ260,pPG611.1,pPG612.1，

腺病毒載體/慢病毒，逆轉(zhuǎn)錄病毒表達(dá)載體及包裝包膜質(zhì)粒，腺病毒系統(tǒng)(Stratagene): pAdEasy-1,pShuttle-CMV,pShuttle,pAdTrack, pAdTrack-CMV, pShuttle-IRES-hrGFP-1、pShuttle-IRES-hrGFP-2、pShuttle-CMV-lacZ, pShuttle-CMV-EGFP-C,pXC1, pBHGE3, 配套大腸桿菌BJ5183,293,293T cell line 腺相關(guān)病毒系統(tǒng)(Stratagene)： pAAV-MCS，pAAV-RC，pHelper，pAAV-LacZ，pAAV-IRES-hrGFP，pCMV-MCS， 慢病毒載體:pLVX-DsRed-Monomer-N1,pLVX-IRES-ZsGreen1,pLVX-AcGFP1-N1,Lenti6/v5-EDST-EGFP,pWPXL, FUGW,pLentilox 3.7,RNAi-Ready pSIREN-Retro Q, RNAi-Ready pSIREN-Retro Q-ZsGreen, pSUPER.Retro-GFP/Neo,pSUPER-Retro-Neo, pSUPER.Retro-puro,PLNCX PLNCX2 pMSCV-HYG pMSCV-neo pMSCV-puro pLEGFP-C1 pLOX-CW-CRE pLOX-GFP-IRES-TK pRetroX-IRES-DsRedExpress, pLVX-IRES-mCherry質(zhì)粒載體。 病毒包裝：二質(zhì)粒包裝系統(tǒng)一： psPAX2 pMD2.G 二質(zhì)粒系統(tǒng)二： pCMV-dR8.2 dvpr pCMV-VSVG 三質(zhì)粒包裝系統(tǒng)具有更高的包裝效率： pMDLg/pRRE pRSV-Rev pMD2.G Invitrogen包裝系統(tǒng)： PLP1 PLP2 PLP/VSVG

基因重組載體敲除載體自殺質(zhì)粒RED重組系統(tǒng)載體pKD3,pKD13,pKD4,pCP20,pKD46, pCVD442,pk18mobsacB,p2NIL, pMAD(革蘭氏陽性菌基因敲除)，pBT2,RN4220,pKC1139,pOJ260, pJQ200SK,pSET152,pWHM10, pk18mobsacB, pGOAL17,pGOAL19,基因打靶載體PL253，PL451，PL452，PL453,配套菌株EL250,EL350,pMLM290,292, KJBAC1,pST1374

熒光蛋白表達(dá)載體熒光定位質(zhì)粒報(bào)告基因載體pEGFP,pEYFP,pECFP,pDsRED, pmCherry,pAcGFP,hrGFP,ZsGreen，pDsRed1-C1, pDsRed1-N1, pDsRED-Monomer-N1, pEYFP-C1, pEYFP-N1, pEGFP-1，pEGFP-C1, pEGFP-N1, pEGFP-N3, pcDNA-EGFP1,pECFP-N1,pECFP-C1, pAcGFP1-1 pDsRED2-ER, pDsRED2-Mito, pDsRED2-Nuc，pDsRED2-Peroxi , pEYFP-Golgi, pEYFP-Mem, pEYFP-ER , pEGFP-Actin, pECFP-ER, pIRES2-EGFP, pLEGFP-C1，pLEGFP-N1，pDsRED-Express-N1,pIRES2-DsRed,pIRES-AcGFP1,pmCherry-C1,N1 啟動(dòng)子增強(qiáng)子報(bào)告載體(Promega，帶有Luc熒光報(bào)告基因)： pGL3-basic，pGL3-control，pGL3-enhancer，pGL3-promoter，pRL-TK，pRL-CMV，pRL-SV40, 信號通路報(bào)告載體(Clontech)： pGAS-TA-Luc，pSTAT3-TA-Luc, pISRE-TA-Luc, pTA-Luc，pIκB-EGFP， pNFAT-TA-Luc，pCaspase3-sensor，pAP1(PMA)-Luc 信號通路報(bào)告載體(Promega)：pGL4.26[luc2P/minP/Hygro],pGL4.29[luc2P/CRE/Hygro],pGL4.30[luc2P/NFAT-RE/Hygro]，pGL4.75 信號通路報(bào)告載體(Stratagene)： p53-Luc，pAP-1-Luc，pNF-κB-Luc，pSRE-Luc， pFA2-Elk1，pFC-MEKK，pFR-luc

昆蟲表達(dá)載體桿狀病毒表達(dá)載體系統(tǒng)pFastBac1,pFastBac1-Gus,pFastBacHT A pFastBacHT-CAT,pFastBacDual,pBlueBacHis2 A DH10Bac配套大腸桿菌,pMIB v5-His B ,pIZT/V5-His，

Taq,Pfu高表達(dá)載體菌株KOD, T4連接酶高表達(dá)質(zhì)粒載體,高產(chǎn)基因工程菌株、酶基因KOD,T4 kinase激酶，Tth反轉(zhuǎn)錄酶MMLV，Klentaq PCR酶，Cre酶，RNA inhibitor抑制酶，DL2000質(zhì)粒

BiFC雙分子熒光表達(dá)載體FRET系統(tǒng)載體pBiFC-VC155，pBiFC-VN173，pBiFC-bFosVC155，pBiFC-bFos(deltaZIP)VC155，pBiFC-bjun-VN173，pCE-BiFC-VN173，pCE-BiFC-VC155，

真菌表達(dá)載體黑曲霉米曲霉黃曲霉表達(dá)質(zhì)粒pBC-hygro,pAN7-1,pBARGPE1, 真菌ATMT農(nóng)桿菌介導(dǎo)轉(zhuǎn)化載體 pBHt1,pBIG2RHPH2-GFP-GUS等，

酵母單雜交三雜交載體，酵母雙雜交系統(tǒng)pGBKT7，pGADT7，pCL1，pGBKT7-53，pGADT7-T，pGBKT7-Lam，配套釀酒酵母AH109 酵母單雜交系統(tǒng)(Clontech):pHISi,pLacZi,pHis2,pHIS2.1 酵母三雜交系統(tǒng):pBridge, YBZ1菌株 Cytotrap Two-Hybrid System(Stratagene): pSos, pSos MAFB, pMyr

噬菌體展示系統(tǒng)載體1.噬菌體展示抗體scFv 質(zhì)粒：pCANTAB5E 宿主菌：TG1 輔助噬菌體：M13K07 可溶表達(dá)菌：HB2151 2.噬菌體展示抗體Fab 質(zhì)粒：pcomb3 宿主菌：XL1-Blue 輔助噬菌體：VCSM13

pEVOL，pBudCE4.1,pBudCE4.1-EGFP,Zeocin,pEGFP-C3,2,1,pmCherry-C1,-N1,pLOVE-miR302/367,pDsRed2-N1,pAcGFP1.1,TALE toolbox kit,pIRES-hyg3,pIRES-Neo3,pIRES-puro3,pSIREN-RetroQ-ZsGreen,GST 14-3-3,pDsRed-Monomer-N1,M50 super8x TOPFlash,pRK793,pCMV-tag5B,pCMV-tag2B，pCMV-N-HA，pUAST,pw-25,PAC-5.1-HISA，pSLIK-Neo,pCMV-myc-cyto,pCMV-myc-nuc,疫苗表達(dá)載體pVAX1,pAdEasy-1,pShuttle,pAdTrack,pAdTrack-CMV,-polyA,pShuttle-EGFP,BJ5183,pXC1,pBHGE3,pSUPER-retro-GFP-Neo,pORF-lacZ，pORF-TK，pCOX2-Luc,pcDNA3.1(-)-myc-His A,pcDNA3.1-p300,pINP, pOprE, pCR2.1 TOPO,pLP1,pLP2,pLP/VSVG,Stbl3,plenti6/V5-DEST,pLVX-shRNA1,2,pLVX-IRES,tdTomato,FUW-teto-hOCT4,hSOX2,hMYC,hKLF4,FUW-M2rtTA,pSecTag2 A,pEYFP-C1,pEGFP-N1,-C1,pcDNA3.1-V5-His A,B,C,pcDNA3.1-EGFP,pLEGFP-C1,α-MHC Promoter,Marc-145猴腎細(xì)胞，Sf9，BmN昆蟲家蠶細(xì)胞，酵母載體pYES2,pYES2.0,INVSc1,pYES3,pYES6,pRS41H,pRS303,304,305，306,pRS424,425,426,414,415,416,pY13,14,15,16,YBZ1,L40酵母菌，pESC-His,pYIP5,pHIS2,pHIS2.1,pHISi,pLacZi,酵母重組載體pUG6,pSH47,植物載體pCAMBIA1300-EGFP, pTCK303,pTRV1,pTRV2,pFGC5941,pUN1301,pSN1301,C58C1，R1601,AGL1農(nóng)桿菌感受態(tài)，原核載體pQE3,pQE30,31,32,pQE60,61,62,M15,DH10Bac,Origami(B)DE3,XL1-Blue,Rosetta gamiB(DE3)pLysS,TKB1,ER2566，DB3.1，JM105,0,DH5a,DH5alpha lambda pir,C43(DE3)菌株感受態(tài)，pTrcHis A,B,C,pTWIN1,pTYB1,pTYB11,pACYC184,pMAL-c5x,c2x,p5x,c4x,pBV220,221,222,pCold I,II,III,TF,pET22a,pET29a,pET28a,pET39b,pET41a,pET42a,p1,pET-sumo,pBADHis A,B,C,pBAD24,34,pETDuet-1,pRSFDuet-1,pCDFDuet-1,pACYCDuet-1,原核熒光pQBI63,pMP2444,鏈球菌表達(dá)、敲除載體pKC1139、pOJ260、pWHM3，pSET152，pVA981，多宿主載體pGOAL19,pBBR1MCS-2,-5,-6,pDSK519,pLA2917，pRK2013，pVLT33,pMAD,RN4220金葡菌株，真菌載體pBARGPE1,pAN7-1,pBHt1,pBIG2RHPH2-GFP-GUS,乳酸菌載體MG1363,pNZ8148,NZ9000,pNZ9530,pMG36e,枯草芽孢桿菌載體pHT43,pWB980,pHT300PLK,pMA5,pUTminiTn5,巨大芽孢桿菌表達(dá)載體pHIS1525,WH320菌,谷氨酸棒狀桿菌表達(dá)載體pEC-XK99E,敲除載體pk18mobsacB,

pET載體

3a, 3d

3c-sumo

5b

11a A+

12a A+

15b A+

16b A+

17b A+

19b A+

20b A+

21a,21b,21d A+

22b A+

23a,23b A+

23a-tev

24a K+

25b K+

26b K+

27b K+

28a,28b,28c K+

28a-sumo

28a-p53

29a K+

30a ,30c K+

31b A+

32a A+

32a pelB A+

35b K+

39b K+

40b K+

41a,41b k+

42a,42b K+

43b A+

43.1a A+

44a A+

48b K+

49b K+

50b K+

pETduet-1

pACYCduet-1

pCDFduet-1

pRSFduet-1

pColAduet-1

pET His

pET DsbA

pET GST

pET Trx

pQE載體

PQE2

pQE9

pQE30,31,32,

pQE30UA

pQE30LacIq

pQE40L

pQE60

pQE70

pQE80L

其他原核表達(dá)載體

PBBR1 MCS Cm+

PBBR1 MCS-2 K+

PBBR1 MCS-3 Tet+

PBBR1 MCS-4 A+

PBBR1 MCS-5 慶大霉素

pMal c2x,p2x,5x,5E

pBV220,221,222

pTrc99a,pTrcCKS，pTrcHis A,B,C

pBAD24,33,43,pBADHis A,B,C,pBADmycHisA

pEZZ18

pThio HisA A+

pGEX4T-1,4T-2，4T-3，5X-1,6p-1,2t,3x

pColdI

pColdII

pColdIII

pColdTF

pTwin1

pRsetA,B,C

PLLP-STQ

pINⅢ-ompA

pkk223-3

pSE380

pJLA502

pMBP-C，pMBP-p

pTYB11,pCYB1

Origene-N-His GST A+

pPin point CAT A+

pPin point xa1 A+

pPin point xa2 A+

pPin point xa3 A+

pCW ori

、

常見克隆載體

puc18,puc19，puc118，puc119

pBlue Script SK+,

pBlue ScriptⅡSK+

pEGM-3ZF(+) A+

pEGM-11ZF(+) A+

pACYC184

pBR322

基因敲除

pKD3,pKD4，pKD46,pcp20

pK18mobSacB

海腎熒光素酶報(bào)告基因

pGL3basic，promotor，enhancer，control

pGL3 CM

pGL4.29,4.30

畢赤酵母表達(dá)載體

pPIC9

pPIC9k

pPIC9k-1

pPIC9k-His

pPIC3.5

pPIC3.5k

pAO815

pHIL-S1

pPICZalphaA，B，C

pPICZalpha D

pPICZalpha E

pPICZalpha GA,GB

pPICZalpha FC

pPICZA，B

pGAPZalphaA，B，C

pMETalphaA,B,C

pFLD-cat A+

pFLD-cat Z+

pFLD1 Z+

畢赤酵母宿主菌

JC220

JC308

GS200

GS190

GS115

KM71

KM71H

Y1991

SMD1168

SMD1168H

X33

釀酒酵母表達(dá)載體

pYES2,pYES2-flag

pYES2NTA,NTB,NTC

pYES3CT

pYIP5,pYRP7,pADH2

pRSH41,pDC315,pDF15,pUG6

pESC-His,BFD14,BFD15,pDNR1,p334,pDR195

pRS316,pRS316HA

YEplac112,YCplac33,YCplac22-EGFP,

YEplac195，YEpLac181,YIP211

pYX212，pYX212-VHB

釀酒酵母宿主菌

INSV-

YPH499

YM4271

BY4741

W303-1A

真核哺乳動(dòng)物熒光載體

pEGFP N1,N2，N3

Origene GFP

pLEGFP N1

pEGFP C1，C3

AIF-EGFP N1

pECFP N1,C1

pEYFP N1,C1

pEYFP-golgi

pEYFPmem

pBFPN1

FIV-BFP

pDsred MonomerN1

pDsRed1 mito

pDsRed2 mito

pDsRed N1，C1

pDsRed2 N1,C1

pDsRed2-ER

mRFP

真核哺乳動(dòng)物載體

pIRES

pIRES2-EGFP

pADeasy-1

pADeasy-1(Bj5183)

pAdtrack

pAdtrack CMV

pShuttle

pShuttle CMV

pRL CMV

pCMV-HA

pVAX1

pVAST

pCI

pCI neo+

pCMV-myc

pflag-CMV2

pcDNA3.1 +

pcDNA3.1 zeo+

pcDNA3.1 neo+

pcDNA3.1 MycHisA

pcDNA3.1 MycHisB

pcDNA3.1-HBV genome

pcDNA3-mRFP

Hsp70-pcDNA3.1

pcDNA 4 Max B

psuper GFP neo

psuper Retro GFP neo

pSilencer4.1CMV puro+

pSilencer4.1CM neo+

psuper GFP neo

psuper retro GFP neo

pdisplay

pBud CE4.1

ptet on, ptet off

pRL-tk，pRL-CMV

植物表達(dá)載體

pcambia1301,1302,1303,1304,1305.2

2300,2301

3300

pcambia1301(EHA105)

pcambia1381Z,1391Z

PBI121，221

PBI221-GFP

植物農(nóng)桿菌宿主

EHA105，LBA4404

植物RNAi載體

PHANNIBAL，PKANNIBAL

啟動(dòng)子檢測

pEGFP-1

pkk232-8

分子伴侶：

PGKJE8，PGro7，pTf16，pKJE7，pGTf2

酵母雙雜交

pGADT7,pGBKT7,pGADT7-T,pGBKlam,pGBK-53,pCL1

AH109,Y187

酵母單雜交

pGAD53m,p53-blue,p53-His,pLacZ,pHis，pHisi-1，

哺乳動(dòng)物雙雜交

pBIND-GFP，pBIND-ID,pGAD424，pGBT9，pG5lac,

大腸桿菌宿主菌

JM101,105,109,110

HB101,K802,C600,TH1,AM1,W3110,DH10bac

Y1089,Y1090,BL21,BL21(DE3),MG1655

BL21(DE3)plysS，BL21AI，BL21SI，BL21codon plus RIPL，BL21 trxB (DE3)，BL21 star

DH5α，DH5αλpir，JM109，JM109(DE3)，0，0F，,K12，DB3.1，XL1-blue，XL-10gold，BM25.8，SG1117，Turbo，TG1，TB1，C41(DE3)，C43(DE3),Turner(DE3),M15，E2566，C2566，ER2529，JF1125，Bj5183,Stbl3,Stbl4

HMS174(DE3), HMS174(DE3)plysS, AD494(DE3)

Novablue(DE3),Rosettalblue(DE3)

Rosetta(DE3),Rosetta(DE5),Rosetta(DE3)plysS,

Orgami(DE3),OrgamiB(DE3),Orgami(DE3)plysS

Rosetta-gami2(DE3)plysS，Rosetta-gami(DE3),

Rosetta-gamiB(DE3),Rosetta-gamiB(DE3)plysS, Rosetta-gami(DE3)placI

枯草表達(dá)系統(tǒng)pMA5,pHCMC05，pGFP22,pGFP315，pHP13,pHP13-43，pHY-43，pWB980,pAOX1

枯草宿主菌：BS168，DB403,BCL,1A75,WB600，WB800N

昆蟲表達(dá)系統(tǒng)：pfastBacI，pfastII，pfastIII，pfastBacHTA，pfastBacHTB，pfastBacHTC，pfastBacdual

巨大芽孢桿菌系統(tǒng)：pHis1525

巨大宿主菌：YYBM1，WH320

乳酸球菌表達(dá)系統(tǒng) pMG36e,NZ9000,MG1363

乳酸酵母表達(dá)系統(tǒng) pKLAC1，GG799

白色念珠菌系統(tǒng)pCR4,pCR4-GFP,p5921

釀酒酵母表面展示系統(tǒng)：pYD1,EBY100

標(biāo)準(zhǔn)微生物菌株

ATCC27853 銅綠假單胞菌

ATCC29212 糞鏈球菌

ATCC29213 金黃色葡萄球菌

ATCC35218 大腸桿菌

ATCC 35098四聯(lián)球菌

NRRL Y-12968熱帶假絲酵母

NRRL Y-2021德巴利酵母

NRRL Y-1603畢赤氏巴斯德酵母

DSM3586野油菜黃